Metabolomics, unbiased global biochemical profiling, is an approach for obtaining a “snapshot” of the metabolism of a population of cells in a bioreactor. As such, it has had particular utility in the area of upstream bioprocess optimization of biopharmaceutical production. This presentation will discuss several case studies from biopharmaceutical collaborations, describing how metabolomics was used to optimize both cell line development and media composition. These case studies will show the monitoring of hundreds of biochemicals and metabolites, found both inside the cell and in the spent media. Using this approach, biopharmaceutical groups have been able to 1) rapidly identify media components which are rate limiting, 2) determine toxic metabolites which build up in either the cell or the spent media, and 3) optimize growth conditions for greatest yield.

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