Generating highly-resolved quantitative profiles of human immune responses remains a significant challenge because clinical samples are often limited in size, and cells of particular interest (e.g., autoreactive T cells) can be rare. This talk will describe an approach for analyzing large populations of single cells for both phenotypic, surface-expressed markers and secreted factors such as cytokines and antigen-specific antibodies. A soft lithographic technique called microengraving is employed to allow concurrent detection of cytokines and antibodies from peripheral blood mononuclear cells. Applications include profiling humoral immune responses, identifying rare antigen-reactive cells, and characterization of functional profiles for lymphocytes.

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