Preliminary Program subject to change
BEC103 Experimental and Computational Tools for Engineering Biomolecules
OverviewMethodological advances have driven dramatic progress in the engineering of proteins and other biomolecules over the last one and one-half decades. While a number of effective and general tools have become available, many biomolecular systems require the development of tailored methods to facilitate functional design or redesign, and substantial opportunities remain for further progress in this field. This session will focus on the development and application of novel methods for engineering biomolecules; contributions describing either experimental or computational methods for rational or evolutionary engineering of biomolecules are welcome. Descriptions of the integration of computational molecular modeling and experimental molecular engineering tools are also encouraged.
Primary SponsorEngineering Functional Biomolecules (BEC1)

Chair

Eric T. Boder
Assistant Professor
Chemical and Biomolecular Engineering
University of Pennsylvania
231 S. 34th Street, 176
Philadelphia, PA 19104
Phone Number: (215) 898-5658
Fax Number: 215-573-6416
Email: boder@seas.upenn.edu

CoChair

Jeffrey J. Gray
Asst. Professor
Johns Hopkins
Chemical and Biomolecular Engineering
3400 N. Charles St.
Baltimore, MD 21218
Phone Number: (410) 516-5313
Fax Number: (410) 516-5510
Email: jgray@jhu.edu
Computational Design of Single-Chain Four-Helix Bundle Proteins That Bind Non-Biological Cofactors
Andreas Lehmann1, Gretchen M. Bender2, H. Christopher Fry1, Donald E. Engel3, Michael J. Therien1, William F. DeGrado2 and Jeffery G. Saven1, (1)Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA 19104, (2)Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, (3)APS Congressional Science Fellow, Office of Representative Rush Holt, Washington, DC 20515
Separation and Identification of Biomolecules Using Nucleic-Acid Amphiphiles in Micellar Electrokinetic Chromatography
James W. Schneider, Carnegie Mellon University, Department of Chemical Engineering, Pittsburgh, PA 15213-3890, Jeffrey M. Savard, Carnegie Mellon University, Dept. of Chemical Engineering, Pittsburgh, PA 15213-3890 and Shane T. Grosser, Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213
Engineering E. Coli for Membrane Protein Biogenesis
A. James Link1, Georgios Skretas1, Nandini S. Aiyappan2 and George Georgiou1, (1)Chemical Engineering, University of Texas at Austin, 1 University Station C0400, Austin, TX 78712, (2)Institute for Cellular and Molecular Biology, University of Texas at Austin, 1 University Station C0400, Austin, TX 78712
Protein Engineering of Gene Switches
Huimin Zhao1, Karuppiah Chockalingam2, Michael McLachlan2 and Ka-chun Lai2, (1)Chemical & Biomolecular Engineering, University of Illinois at Urbana-Champaign, 215 RAL, Box C-3, MC-712, 600 S. Mathews Ave., Urbana, IL 61801, (2)University of Illinois at Urbana-Champaign, 215 RAL, Box C-3, MC-712, 600 S. Mathews Ave., Urbana, IL 61801
Computationally Mapping Sequence Space for Evolutionary Protein Design
Kathryn A. Armstrong, Biological Engineering, Massachusetts Institute of Technology, 32 Vassar St., 32-211, Cambridge, MA 02139 and Bruce Tidor, Biological Engineering; Electrical Engineering & Computer Science, MIT, Cambridge, MA 02139
Simple Conjugation and Purification of Quantum Dot-Antibody Complexes Using a Thermally Responsive Elastin-Protein L Scaffold as Immunofluoresecent Agents
Wilfred Chen, University of California, Riverside, A242 Bourns, Riverside, CA 92521, Ashok Mulchandani, Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521 and U. Loi Lao, Chemical and Environmental Engineering, University of California, 900 University Avenue, Riverside, CA 92521
Novel High-Throughput Suface Plasmon Resonance Methodology for Proteomics
Di Gao, Department of Chemical Engineering, University of Pittsburgh, Pittsburgh, PA 15261 and Jerome Schultz, Bioengineering, University of California, Riverside, Bourns Hall, Room A247, Rivreside, CA 92521
Protein Engineering in Biomedicine
K. Dane Wittrup, Chemical Engineering and Biological Engineering, Massachusetts Institute of Technology, E19-551, Cambridge, MA 02139
Redesigning Complement 3a and an HIV-1 Gp41 Inhibitor Using a New De Novo Protein Design Approach
Ho Ki Fung, Department of Chemical Engineering, Princeton University, Engineering Quadrangle, Olden Street, Princeton, NJ 08544 and Christodoulos A. Floudas, Chemical Engineering, Princeton University, Dept of Chemical Engineering; A215 Engineering Quadrangle, Princeton, NJ 08544
Designed Divergent Evolution of Enzyme Function
Yasuo Yoshikuni, Bioengineering, University of California, Berkeley, 717 Potter St., Berkeley, CA 94704, Japan and Jay D. Keasling, Chemical Engineering, University of California, Berkeley, Berkeley, CA 94720
Design and Evaluation of Computationally-Inspired Protein Combinatorial Library Methods
Stephen L. Mayo, Division of Biology, Division of Chemistry and Chemical Engineering, California Institute of Technology, Caltech 114-96, 1200 E. California Blvd, Pasadena, CA 91125-9600
Prediction and Design of Macromolecular Structures and Interactions
David Baker, Department of Biochemistry, University of Washington, J Wing, Health Sciences Building, Box 357350, Seattle, WA 98195
Chemical Complementation: a Genetic Selection System in Yeast for Protein and Enzyme Engineering and Drug Discovery
Bahareh Azizi, Chemistry and Biochemistry, Georgia Institute of Technology, 315 FERST DR. #3432, Atlanta, GA 303332 and Donald F. Doyle, Georgia Institute of Technology, 315 FERST DR. #3432, Atlanta, GA 303332
Engineering New Molecular Sensors and Switches for Programming Cellular Systems
Maung Nyan Win and Christina Smolke, Chemistry and Chemical Engineering, California Institute of Technology, 236A Spalding, Mail Code 210-41, Pasadena, CA 91125

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